Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling

• DOI: 10.1016/j.celrep.2019.05.106
• Publication Date: 2019-07-02T14:37:16Z
• Authors: Arjan Hada; Swetansu K. Hota; Jie Luo; Yuan-chi Lin; Seyit Kale; Alexey K. Shaytan; Saurabh K. Bhardwaj; Jim Persinger; Jeff Ranish; Anna R. Panchenko; Blaine Bartholomew
• Keywords: Histone octamer; Histone code; Chromatosome; Linker DNA

Nucleosomes are the fundamental building blocks of chromatin that regulate DNA access and composed histone octamers. ATP-dependent remodelers like ISW2 by translationally moving nucleosomes to different regions. We find octamers more pliable than previously assumed distorted early in remodeling before enters ATPase motor moves processively on nucleosomal DNA. Uncoupling activity from nucleosome movement with deletion SANT domain C terminus Isw2 catalytic subunit traps intermediates which octamer structure is changed. restricting chemical crosslinking also resembling those seen loss domain. Other evidence shows intrinsically prone changing their conformation can be merely H3-H4 tetramer disulfide crosslinking.