Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice

• DOI: 10.1016/j.celrep.2019.06.062
• Publication Date: 2019-07-23T10:38:47Z
• Authors: Agostina Carestia; Hebe A. Mena; Cinthia M. Olexen; Juan M. Ortiz Wil...; Soledad Negrotto; Andrea E. Errasti; Ricardo M. Gómez; Craig N. Jenne; Eugenio A. Carrer...; Mirta Schattner
• Keywords: CD163; Macrophage polarization

We investigated the contribution of human platelets to macrophage effector properties in presence lipopolysaccharide (LPS), as well beneficial effects and time frame for platelet transfusion septic animals. Our results show that sequester both pro-(TNF-α/IL-6) anti-(IL-10) inflammatory cytokines released by monocytes. Low LPS concentrations (0.01 ng/mL) induced M2 polarization decreasing CD64 augmenting CD206 CD163 expression; yet, skewed monocytes toward type 1 (M1) phenotype a cell-contact-dependent manner glycoprotein Ib (GPIb)-CD11b axis. Accordingly, platelet-licensed macrophages showed increased TNF-α levels, bacterial phagocytic activity, reduced healing capability. Platelet inducible nitric oxide synthase (iNOS)+ macrophages, improving clearance survival rates mice up 6 h post-infection, an effect was abolished CD11b GPIb blockade. demonstrate orchestrate responses, clinical outcome sepsis narrow but relevant frame.