Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina

• DOI: 10.1016/j.celrep.2019.10.084
• Publication Date: 2019-11-26T15:50:05Z
• Authors: Diana Pauly; Divyansh Agarwal; Nicholas Dana; Nicole Schäfer; Josef Biber; Kirsten A. Wunder...; Yassin Jabri; Tobias Straub; Nancy R. Zhang; Avneesh K. Gautam; Bernhard H.F. Weber; Stefanie M. Hauck; Mijin Kim; Christine A. Curcio; Dwight Stambolian; Mingyao Li; Antje Grosche
• Keywords: Cell type

Complement dysregulation is a feature of many retinal diseases, yet mechanistic understanding at the cellular level limited. Given this knowledge gap about which cells express complement, we performed single-cell RNA sequencing on ∼92,000 mouse and validated our results in five major purified cell types. We found evidence for distributed cell-type-specific complement expression across 11 Notably, Müller are contributor activators c1s, c3, c4, cfb. Retinal pigment epithelium (RPE) mainly expresses cfh terminal components, whereas cfi cfp transcripts most abundant neurons. Aging enhances cfb, cfp, expression, while decreases. Transient ischemia increases microglia, cells, RPE. In summary, report unique signature murine types suggesting well-orchestrated regulation local microenvironment.