Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans

• DOI: 10.1016/j.celrep.2020.108684
• Publication Date: 2021-02-10T02:34:25Z
• Authors: Henry J. Sutton; Racheal Aye; Azza H. Idris; Rachel Vistein; Eunice Nduati; Oscar Kai; Jedida Mwacharo; Xi Li; Xin Gao; T. Daniel Andrews; Marios Koutsakos; Thi H.O. Nguyen; Maxim Nekrasov; Peter Milburn; Auda Eltahla; Andrea A. Berry; Natasha KC; Sumana Chakravarty; B. Kim Lee Sim; Adam K. Wheatley; Stephen J. Kent; Stephen L. Hoffman; Kirsten E. Lyke; Philip Bejon; Fabio Luciani; Katherine Kedzierska; Robert A. Seder; Francis M. Ndungu; Ian A. Cockburn
• Keywords: Lineage (genetic)

The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the both antigen-specific and total in healthy subjects malaria-exposed individuals. This reveals two cell lineages: a classical lineage activated resting memory an alternative lineage, which includes previously described atypical cells. Although have been associated with disease states, common controls, as well further track Plasmodium-specific after malaria vaccination naive volunteers. find that are primed initial immunization respond booster doses. However, develop phenotype repeated boosts. data highlight part wider normal component immune responses.