High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature

• DOI: 10.1016/j.celrep.2021.108806
• Publication Date: 2021-03-02T17:15:47Z
• Authors: James T. Harden; Xi Wang; Jiaying Toh; Adam X. Sang; Ryanne A. Brown; Carlos O. Esquivel; Olivia M. Martinez; Sheri M. Krams
• Keywords: Signature (topology)

Alloimmune responses in acute rejection are complex, involving multiple interacting cell types and pathways. Deep profiling of these has been limited by technology that lacks the capacity to resolve this high dimensionality. Single-cell mass cytometry is used characterize alloimmune response early rejection, measuring 37 parameters simultaneously, across time points two models: a murine cardiac vascularized composite allotransplant (VCA). Semi-supervised hierarchical clustering group related defined combinatorial expression surface intracellular proteins, along with markers effector function activation. This profile mapped visualize changes antigen composition types, revealing phenotypic signatures T cells, natural killer (NK) myeloid subsets conserved firmly distinguish rejecting from non-rejecting grafts. These data provide comprehensive, high-dimensional cellular after allograft transplantation.