Regional specialization and fate specification of bone stromal cells in skeletal development

0301 basic medicine osteoblast lineage cells Transcription, Genetic QH301-705.5 adipocytes Article Bone and Bones Receptor, Platelet-Derived Growth Factor beta Mice 03 medical and health sciences diaphysis Animals Cell Lineage Biology (General) metaphysis 0303 health sciences Bone Development Endothelial Cells Cell Differentiation Mesenchymal Stem Cells bone mesenchymal stromal cells mouse genetics Mice, Inbred C57BL Animals, Newborn Cardiovascular and Metabolic Diseases Organ Specificity Single-Cell Analysis Stromal Cells Signal Transduction
DOI: 10.1016/j.celrep.2021.109352 Publication Date: 2021-07-13T15:05:51Z
ABSTRACT
Bone stroma contributes to the regulation of osteogenesis and hematopoiesis but also to fracture healing and disease processes. Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous mixture of different subpopulations with distinct molecular and functional properties. The lineage relationship between BMSC subsets and their regulation by intrinsic and extrinsic factors are not well understood. Here, we show with mouse genetics, ex vivo cell differentiation assays, and transcriptional profiling that BMSCs from metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct. Fate-tracking experiments and single-cell RNA sequencing indicate that bone-forming osteoblast lineage cells and dpMSCs, including leptin receptor-positive (LepR+) reticular cells in bone marrow, emerge from mpMSCs in the postnatal metaphysis. Finally, we show that BMSC fate is controlled by platelet-derived growth factor receptor β (PDGFRβ) signaling and the transcription factor Jun-B. The sum of our findings improves our understanding of BMSC development, lineage relationships, and differentiation.
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