PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

• DOI: 10.1016/j.celrep.2022.111114
• Publication Date: 2022-07-19T22:27:49Z
• Authors: Diana Sá da Bandeira; Alastair Morris K...; Madalena Marques; Mario Gomez-Salazar; Telma Ventura; Zaniah Nashira Go...; Dorota Stefancova; Fiona Rossi; Matthieu Vermeren; Chris Sebastiaan ...; Mariana Beltran; Neil Cowan Henderson; Bongnam Jung; Reinier van der L...; Harmen Jan George...; Wilfred F.J. van ...; Christer Betsholtz; Stuart John Forbes; Henar Cuervo; Mihaela Crisan
• Keywords: Dorsal aorta; Hematopoietic stem cell

Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor (HSPC) and is required niche. Little known about murine HSPC vivo whether involved. Here, we show that PDGFRβ expressed distinct perivascular stromal layers mid-gestation dorsal aorta, its deletion impairs hematopoiesis. We demonstrate PDGFRβ+ cells play a dual role They act aortic niche to support HSPCs, addition, embryonic precursors give rise subset of HSPCs persist into adulthood. These findings provide crucial information for controlled production vitro.