PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

• DOI: 10.1016/j.celrep.2022.111114
• Publication Date: 2022-07-19T22:27:49Z
• Authors: Diana Sá da Bandeira; Alastair Morris K...; Madalena Marques; Mario Gomez-Salazar; Telma Ventura; Zaniah Nashira Go...; Dorota Stefancova; Fiona Rossi; Matthieu Vermeren; Chris Sebastiaan ...; Mariana Beltran; Neil Cowan Henderson; Bongnam Jung; Reinier van der L...; Harmen Jan George...; Wilfred F.J. van ...; Christer Betsholtz; Stuart John Forbes; Henar Cuervo; Mihaela Crisan
• Keywords: 0301 basic medicine; Cellbiologi; Cell- och molekylärbiologi; Cell Biology; Hematopoietic Stem Cells; Article; Hematopoiesis; Receptor, Platelet-Derived Growth Factor beta; Mice; 03 medical and health sciences; 0302 clinical medicine; Mesonephros; Animals; Stromal Cells; Cell and Molecular Biology; Zebrafish

Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.