PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

0301 basic medicine Cellbiologi Cell- och molekylärbiologi Cell Biology Hematopoietic Stem Cells Article Hematopoiesis Receptor, Platelet-Derived Growth Factor beta Mice 03 medical and health sciences 0302 clinical medicine Mesonephros Animals Stromal Cells Cell and Molecular Biology Zebrafish
DOI: 10.1016/j.celrep.2022.111114 Publication Date: 2022-07-19T22:27:49Z
ABSTRACT
Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.
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