Temporal analysis suggests a reciprocal relationship between 3D chromatin structure and transcription

• DOI: 10.1016/j.celrep.2022.111567
• Publication Date: 2022-11-01T14:43:38Z
• Authors: Kathleen S.M. Reed; Eric S. Davis; Marielle L. Bond; Alan Cabrera; Eliza Thulson; Ivana Yoseli Quiroga; Shannon Cassel; Kamisha T. Woolery; Isaac Hilton; Hyejung Won; Michael I. Love; Douglas H. Phanstiel
• Keywords: Reciprocal; Transcription

To infer potential causal relationships between 3D chromatin structure, enhancers, and gene transcription, we mapped each feature in a genome-wide fashion across eight narrowly spaced time points of macrophage activation. Enhancers genes connected by loops exhibit stronger correlations histone H3K27 acetylation expression than can be explained genomic distance or physical proximity alone. At these looped enhancer-promoter pairs, changes at distal enhancers precede expression. Changes directional bias differential loop anchors; gained are associated with increased oriented away from the center loop, lost often accompanied high levels transcription within boundaries themselves. These results consistent reciprocal relationship where facilitate connecting promoters to whereas impede formation.