Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo

• DOI: 10.1016/j.celrep.2023.112382
• Publication Date: 2023-04-14T09:26:11Z
• Authors: Lauren Forbes Beadle; Hongpeng Zhou; Magnus Rattray; Hilary L. Ashe
• Keywords: Male; 0303 health sciences; X Chromosome; QH301-705.5; MS2; burst amplitude; live imaging; Drosophila embryo; Article; X chromosome; 03 medical and health sciences; Dosage Compensation, Genetic; dosage compensation; transcriptional bursting; Animals; Drosophila Proteins; RNA polymerase II; CP: Molecular biology; Female; Drosophila; Biology (General); Transcription Factors

SummaryDosage compensation, the balancing of X linked gene expression between sexes and to the autosomes, is critical to an organism’s fitness and survival. InDrosophila, dosage compensation involves hypertranscription of the male X chromosome. Here we use quantitative live imaging and modelling at single-cell resolution to determine the mechanism underlying X chromosome dosage compensation inDrosophila. We show that the four X chromosome genes studied undergo transcriptional bursting in male and female embryos. Mechanistically our data reveal that transcriptional upregulation of male X chromosome genes is primarily mediated by a higher RNA polymerase II initiation rate and burst amplitude across the expression domain. In contrast, burst frequency is spatially modulated in nuclei within the expression domain in response to different transcription factor concentrations to tune the transcriptional response. Together, these data show how the local and global regulation of distinct burst parameters establish the complex transcriptional outputs underpinning developmental patterning.