Modeling human ectopic pregnancies with trophoblast and vascular organoids

• DOI: 10.1016/j.celrep.2023.112546
• Publication Date: 2023-05-23T11:24:00Z
• Authors: Xiaoya Zhao; Zhenwu Zhang; Qian Zhu; Yurui Luo; Qinying Ye; Shuxiang Shi; Xueyang He; Jing Zhu; Duo Zhang; Wei Xia; Yiqin Zhang; Linlin Jiang; Long Cui; Yinghui Ye; Yangfei Xiang; Junhao Hu; Jian Zhang; Chao-Po Lin
• Keywords: Trophoblast; Vasculogenesis; Organoid; Ectopic expression; Progenitor

Ruptured ectopic pregnancy (REP), a complication caused by aberrant implantation, deep invasion, and overgrowth of embryos in fallopian tubes, could lead to rupture tubes accounts for 4%-10% pregnancy-related deaths. The lack phenotypes rodents hampers our understanding its pathological mechanisms. Here, we employed cell culture organoid models investigate the crosstalk between human trophoblast development intravillous vascularization REP condition. Compared with abortive (AEP), size placental villi depth invasion are correlated extent vascularization. We identified key pro-angiogenic factor secreted trophoblasts, WNT2B, that promotes villous vasculogenesis, angiogenesis, vascular network expansion Our results reveal important role WNT-mediated angiogenesis an co-culture model investigating intricate communications trophoblasts endothelial/endothelial progenitor cells.