rRNA intermediates coordinate the formation of nucleolar vacuoles in C. elegans

• DOI: 10.1016/j.celrep.2023.112915
• Publication Date: 2023-08-02T00:47:48Z
• Authors: Demin Xu; Xiangyang Chen; Yan Kuang; Minjie Hong; Ting Xu; Ke Wang; Xinya Huang; Chuanhai Fu; Ke Ruan; Chengming Zhu; Xuezhu Feng; Shouhong Guang
• Keywords: Fibrillarin; Nucleoplasm; Nucleolin; Transcription; RNA polymerase I; ribosome biogenesis

The nucleolus is the most prominent membraneless organelle within nucleus. How nucleolar structure regulated poorly understood. Here, we identified two types of nucleoli in C. elegans. Type I are spherical and do not have visible vacuoles (NoVs), rRNA transcription processing factors evenly distributed throughout nucleolus. II contain vacuoles, exclusively accumulate periphery rim. NoV contains nucleoplasmic proteins capable exchanging contents with nucleoplasm. high-order dynamically Faithful important to prohibit NoVs. depletion 27SA2 resulted formation. inhibition RNA polymerase (RNAPI) conserved factors, nucleolin fibrillarin, prohibits formation This finding provides a mechanism coordinate maintenance gene expression.