BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

• DOI: 10.1016/j.celrep.2023.112938
• Publication Date: 2023-08-06T21:04:45Z
• Authors: Tongbing Su; Weihong Wang; Zheng Wang; Peirong Li; Xiaoyun Xin; Yangjun Yu; Deshuang Zhang; Xiuyun Zhao; Jiao Wang; Liling Sun; Guihua Jin; Fenglan Zhang; Shuancang Yu
• Keywords: Brassica rapa; Verticillium wilt; Verticillium

Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW have been identified by forward genetic approaches, resulting in limited application breeding. We combine genome-wide association study natural population and quantitative trait locus mapping an F2 identify that the MYB transcription factor BrMYB108 regulates VW. A 179 bp insertion promoter alters its expression pattern during longisporum (VL) infection. High leads high VL resistance, confirmed disease tests using overexpression loss-of-function mutants. Furthermore, we verify confers regulating reactive oxygen species (ROS) generation through binding promoters respiratory burst oxidase (Rboh). mutant AtRbohF Arabidopsis shows significant susceptibility VL. Thus, target ROS could be used as targets engineering B. rapa.