The genome of Stephania japonica provides insights into the biosynthesis of cepharanthine

• DOI: 10.1016/j.celrep.2024.113832
• Publication Date: 2024-02-20T21:54:15Z
• Authors: Zhuo Liu; Shaoqin Shen; Yujie Wang; Shuqi Sun; Tong Yu; Yanhong Fu; Rong Zhou; Chunjin Li; Rui Cao; Yanshu Zhang; Nan Li; Liangdan Sun; Xiaoming Song
• Keywords: CP: Genomics; CP: Plants; 0303 health sciences; QH301-705.5; Karyotype; COVID-19; karyotype reconstruction; Benzylisoquinolines; S. japonica; 03 medical and health sciences; genome assembly and evolution; Genome Size; database construction; cepharanthine; Benzodioxoles; Biology (General); benzylisoquinoline alkaloid; Phylogeny; Stephania

Stephania japonica is an early-diverging eudicotyledon plant with high levels of cepharanthine, proven to be effective in curing coronavirus infections. Here, we report a high-quality S. genome. The genome size 688.52 Mb, and 97.37% sequences anchor 11 chromosomes. comprises 67.46% repetitive 21,036 genes. It closely related two Ranunculaceae species, which diverged from their common ancestor 55.90–71.02 million years ago (Mya) whole-genome duplication 85.59–96.75 Mya. We further reconstruct ancestral karyotype Ranunculales. Several cepharanthine biosynthesis genes are identified verified by western blot. Two (Sja03G0243 Sja03G0241) exhibit catalytic activity as shown liquid chromatography-mass spectrometry. Then, genes, transcription factors, CYP450 family used construct comprehensive network. Finally, eudicotyledonous resources (EEGR) database. As the first Menispermaceae released, this study provides rich for genomic studies.