IQGAP1 and NWASP promote human cancer cell dissemination and metastasis by regulating β1-integrin via FAK and MRTF/SRF

• DOI: 10.1016/j.celrep.2024.113989
• Publication Date: 2024-03-26T20:10:12Z
• Authors: Camilla Cerutti; Serena Lucotti; Sofia T. Menendez; Nicolas Reymond; Ritu Garg; Ignacio A. Romero; Ruth Muschel; Anne J. Ridley
• Keywords: IQGAP1

Attachment of circulating tumor cells to the endothelial (ECs) lining blood vessels is a critical step in cancer metastatic colonization, which leads outgrowth. Breast and prostate cancers are common malignancies women men, respectively. Here, we observe that β1-integrin required for human breast cell adhesion ECs under shear-stress conditions vitro lung vessel vivo. We identify IQGAP1 neural Wiskott-Aldrich syndrome protein (NWASP) as regulators transcription expression cells. NWASP depletion decreases retention vasculature nodule formation Mechanistically, act downstream Cdc42 increase both via extracellular signal-regulated kinase (ERK)/focal signaling at level by myocardin-related factor/serum response factor (SRF) transcriptionally. Our results potential therapeutic targets reduce early dissemination.