Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

• DOI: 10.1016/j.celrep.2024.114311
• Publication Date: 2024-06-05T20:47:54Z
• Authors: Padma P. Nanaware; Zohaib N. Khan; Cristina C. Clement; Madhur Shetty; Ines Mota; Ethan S. Seltzer; Monika Dzieciatko...; Fabia Gamboni; Angelo D’Alessandro; Charles Ng; Manabu Nagayama; Cheryl F. Lichti; Rajesh K. Soni; Jacob B. Geri; Irina Matei; David Lyden; Randy Longman; Theresa T. Lu; Xiaoxiao Wan; Emil R. Unanue; Lawrence J. Stern; Laura Santambrogio
• Keywords: Immunosurveillance

The lymphatic fluid is the conduit by which part of tissue "omics" transported to draining lymph node for immunosurveillance. Following cannulation pre-nodal cervical and mesenteric afferent lymphatics, herein we investigate proteomic composition, uncovering that its composition varies according origin. Tissue specificity also reflected in dendritic cell-major histocompatibility complex class II-eluted immunopeptidome harvested from nodes. inflammatory disruption gut barrier, antigenic loads are analyzed both mice subjects with bowel diseases. Gastrointestinal damage reflects damage-associated molecular pattern signatures, microbiome-derived by-products, immunomodulatory molecules, including metabolites gut-brain axis, mapped lymph. Our data point relevance probe tissue-specific load