Getting more out of the zebrafish light dark transition test

• DOI: 10.1016/j.chemosphere.2022.133863
• Publication Date: 2022-02-03T21:01:12Z
• Authors: Ann-Cathrin Haigis; Richard Ottermanns; Andreas Schiwy; Henner Hollert; Jessica Legradi
• Keywords: Danio

In (eco-)toxicological studies the light/dark transition (LDT) test is one of most frequently used behaviour assays with zebrafish eleutheroembryos. However, study results vary regarding data presentation and analysis mostly focus on a limited amount recorded data. this study, we investigated whether monitoring two behavioural outcomes (time distance moved) together analysing multiple parameters can improve sensitivity interpretation. As proof principle 5-day old (Danio rerio) eleutheroembryos exposed to either endocrine disruptors (EDs) or acetylcholine esterase (AChE) inhibitors were investigated. We analysed conventional such as mean sum implemented additional endpoints minimum maximum moved new assessing bursting response Furthermore, changes in eleutheroembryonic during moment light dark added. To control-normalised displayed radar charts, enabling simultaneous different relation each other. This enabled us identify relevant certain response. A cut off threshold using control was applied that altered biological manner. Our approach able detect effects remained undetected when done bar graphs - cases averaged, values. By combining charts by control-based thresholds, increase promote deeper understanding LDT thereby increased its usability for toxicity studies.