Microbead beating extraction of avian eggs for polycyclic aromatic compounds

• DOI: 10.1016/j.chemosphere.2023.139059
• Publication Date: 2023-05-31T09:31:45Z
• Authors: Zhe Xia; Ifeoluwa Idowu; Thor Halldorson; Amica-Mariae Lucas; Claire Stein; Manpreet Kaur; Thane Tomy; Chris Marvin; Philippe J. Thomas; Craig E. Hebert; Reyd A. Smith; Frederic Dwyer-Sa...; Jennifer F. Prove...; Gregg T. Tomy
• Keywords: Microbead (research)

Due to their relatively high trophic position and importance as a food source for many communities in the circumpolar north, seabird eggs are an important matrix for monitoring contaminant levels. In fact, many countries, including Canada, have established long-term seabird egg contaminant monitoring programs, with oil related compounds a contaminant of emerging concern for seabirds in several regions. Current approaches to measuring many contaminant burdens in seabird eggs are time-consuming and often require large volumes of solvent. Here we propose an alternative approach, based on the principle of microbead beating tissue extraction using custom designed stainless-steel extraction tubes and lids, to measure a suite of 75 polycyclic aromatic compounds (polycyclic aromatic hydrocarbons (PAHs), alkyl-PAHs, halogenated-PAHs and some heterocyclic compounds) comprising a wide-range of chemical properties. Our method was conducted in strict accordance with ISO/IEC 17025 guidelines for method validation. Accuracies for our analytes generally ranged from 70 - 120%, and intra and inter-day repeatability for most analytes were < 30%. Limits of detection/quantitation for the 75 target analytes were < 0.2/0.6 ng g-1. The level of contamination in our method blanks was significantly smaller in our stainless-steel tubes/lids relative to commercially available high-density plastic alternatives. Overall, our method meets our data quality objectives and results in a notable reduction in sample processing times relative to current approaches.