Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system

• DOI: 10.1016/j.cj.2020.02.005
• Publication Date: 2020-03-26T00:04:37Z
• Authors: Shiyu Wang; Yihao Yang; Min Guo; Chongyuan Zhong; Changjie Yan; Shengyuan Sun
• Keywords: Japonica rice; Wild type

High grain protein content (GPC) reduces rice eating and cooking quality (ECQ). We generated OsAAP6 OsAAP10 knockout mutants in three high-yielding japonica varieties one line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background T0 generation, GPC T1 generation decreased significantly, owing mainly to a reduction glutelin content. Amylose was down-regulated significantly some Osaap6 all Osaap10 mutants. The increased taste value of these supported by Rapid Visco Analysis (RVA) profiles, which showed higher peak viscosity breakdown lower setback than wild type. There were no significant deficiencies agronomic traits Targeted mutagenesis OsAAP10, especially system can rapidly reduce improve ECQ rice, providing new strategy for breeding cultivars desired ECQ.