OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice (Oryza sativa L.)

• DOI: 10.1016/j.cj.2020.12.005
• Publication Date: 2021-01-26T02:54:15Z
• Authors: Jiaming Li; Minghui Zhang; Luomiao Yang; Xinrui Mao; Jinjie Li; Lu Li; Jingguo Wang; Hualong Liu; Hongliang Zheng; Zichao Li; Hongwei Zhao; Xianwei Li; Lei Lei; Jian Sun; Detang Zou
• Keywords: Drought Tolerance

The C (Cys) 2H (His) 2-type transcription factor is one of the most important factors in plants and plays a regulatory role physiological responses rice to abiotic stresses. A novel C2H2-type zinc finger protein, abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3), was found confer drought stress tolerance by enhancing antioxidant defense regulating OsGPX1. Overexpression OsADR3 increased increasing ROS scavenging ability ABA sensitivity. In contrast, CRISPR/Cas9-mediated knockout osadr3 sensitivity oxidative stress. An exogenous ROS-scavenging reagent restored drought-stress osadr3-CRISPR plants. Global transcriptome analysis suggested that expression OsGPX1 under Electrophoretic mobility shift, yeast one-hybrid, dual-luciferase reporter assays revealed modified directly binding its promoter. Knockdown repressed OsADR3-overexpression These findings suggest positive inducing associated with signaling pathway rice.