The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice

• DOI: 10.1016/j.cj.2022.03.004
• Publication Date: 2022-04-19T17:18:06Z
• Authors: Qingwen Wang; Lan Ni; Zhenzhen Cui; Jingjing Jiang; Chao Chen; Mingyi Jiang
• Keywords: Homeostasis

Rice (Oryza sativa L.) is a staple cereal for more than two thirds of the world's population. Soil salinity severely limits rice growth, development, and grain yield. It desirable to elucidate mechanism rice's salt-stress response. As major source H2O2, NADPH oxidase (Rboh) believed be involved in tolerance. However, function Rboh salt stress response remain unclear. In this study, we found that expression OsRbohA was up-regulated by NaCl treatment shoots roots seedlings. Knockout reduced tolerance stress. blocked NaCl-induced increases activity H2O2 content roots. knockout inhibited root growth disrupted K+ homeostasis reducing transporters channel-associated genes (OsGORK, OsAKT1, OsHAK1, OsHAK5) under treatment. Under treatment, also subcellular contents plasma membrane soluble fraction. Overexpression increased loss ions These results indicate OsRbohA-mediated accumulation modulates homeostasis, thereby increasing rice.