Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver

• DOI: 10.1016/j.cmet.2016.10.003
• Publication Date: 2016-11-03T17:17:01Z
• Authors: Jingkui Wang; Daniel Mauvoisin; Eva Martin; Florian Atger; Antonio Núñez Gal...; Loïc Dayon; Federico Sizzano; Alessio Palini; Martin Kussmann; Patrice Waridel; Manfredo Quadroni; Vjekoslav Dulić; Felix Naef; Frédéric Gachon
• Keywords: ribosome biogenesis

Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at transcriptional level, much less is known about temporally functions within nucleus protein level. Here, we quantified temporal nuclear accumulation proteins phosphoproteins from mouse liver SILAC proteomics. We identified around 5,000 proteins, over 500 which showed a diurnal accumulation. Parallel analysis phosphoproteome enabled inference activity kinases accounting for rhythmic phosphorylation. Many were parts complexes involved in regulation, ribosome biogenesis, DNA repair, cell cycle potentially associated hepatocyte polyploidy. Taken together, these findings provide unprecedented regulatory landscape nucleus.