Prevalence and transmission dynamics of Escherichia coli ST131 among contacts of infected community and hospitalized patients

• DOI: 10.1016/j.cmi.2017.09.007
• Publication Date: 2017-09-20T11:02:25Z
• Authors: E. Torres; L. López-Cerero; I. Morales; M.D. Navarro; J. Rodríguez-Baño; A. Pascual
• Keywords: Male; 0301 basic medicine; Cross Infection; 0303 health sciences; ST131; Intestinal persistence; Microbial Sensitivity Tests; Intestinal carriage; 3. Good health; Community-Acquired Infections; Hospitalization; Feces; 03 medical and health sciences; Spain; Drug Resistance, Multiple, Bacterial; Urinary Tract Infections; Escherichia coli; Prevalence; Humans; Female; Longitudinal Studies; Horizontal transmission; Escherichia coli Infections

The Escherichia coli O25b-associated ST131 clonal group was recently found to be prevalent in our area as a cause of community-acquired urinary tract infections. We evaluated the transmission dynamics and longitudinal persistence of E. coli O25b-ST131 between patients with nosocomial and community-acquired infections and their contacts.Prevalence and transmission of O25b/pabB3/B23 isolates were compared in 38 community clusters, 30 nosocomial clusters and 50 healthy volunteers. Duration of colonization was studied at 1 to 4 months and 6 to 12 months after the first sample. Isolates exhibiting a three-band or less difference by pulsed-field gel electrophoresis were assigned to the same pulsotype.Colonization was found to be more frequent in index cases (31/68, 45.6%) than in contacts (25/118, 21.2%; p 0.0009) or volunteers (1/50, 2%; p 0.0009). Seven (11%) of 64 isolates were extended-spectrum β-lactamase producers. Transmission occurred in 61% (8/13) community clusters and in 12% (1/8) nosocomial clusters. Thirteen (56.5%) of the 23 initial carriers assessed at 1 to 4 months remained colonized. Only 2 (13.3%) of 15 positive patients followed for 6 to 12 months showed prolonged carriage, and none was infected with extended-spectrum β-lactamase producers. Six previously positive individuals acquired a different ST131 pulsotype (5/23 at sample 2 and 1/15 at sample 3), and three previously negative individuals became positive (2/46 at 1-4 months and 1/33 at 6-12 months).Person-to-person transmission or acquisition from a common source of E. coli O25b-associated ST131 is more frequent in the household setting than in the nosocomial setting. The carrier state does not usually last beyond 4 months, with new acquisitions in certain individuals.