Numerical evaluation and experimental validation of fluid flow behavior within an organ-on-a-chip model

• DOI: 10.1016/j.cmpb.2023.107883
• Publication Date: 2023-10-28T02:02:37Z
• Authors: Violeta Carvalho; Inês M. Gonçalves; Nelson Rodrigues; Paulo Sousa; Vânia Pinto; Graça Minas; Hirokazu Kaji; Su Ryon Shin; Raquel O. Rodrigues; Senhorinha F.C.F....; Rui A. Lima
• Keywords: Experimental data

By combining biomaterials, cell culture, and microfluidic technology, organ-on-a-chip (OoC) platforms have the ability to reproduce physiological microenvironment of human organs. For this reason, these advanced devices been used resemble various diseases investigate novel treatments. In addition experimental assessment, numerical studies biodevices performed aiming at their improvement optimization. Despite considerable progress in modeling biodevices, validation computational models through comparison with assays remains a significant gap current literature. This step is critical ensure accuracy reliability models, consequently enhance confidence predictive results. The aim present work develop model capable reproducing fluid flow behavior within an OoC, for future investigations, encompassing geometry optimization.In study, OoC device was undertaken. comprised both quantitative qualitative assessments trace microparticles flowing physical model. High-speed microscopy images flow, using blood analog fluid, were analyzed compared simulations run Ansys Fluent software. analysis, particles' paths inlet bifurcations observed whereas, particle velocities measured. Furthermore, oxygen transport simulated evaluated different Reynolds numbers.In analyses, results predicted by ones outputted good agreement. These findings underscore capability potential developed examination vertical positions organoid has revealed that lower positions, predominantly occurs diffusion, leading symmetric distribution oxygen. Contrastingly, convection phenomenon becomes more evident upper region organoid.The successful against data shows its simulating which can expedite design process reducing need prototypes' fabrication costly laboratory experiments.