Substrate stiffness dominants cell gene expression via regulation of HDAC3 subcellular localization

• DOI: 10.1016/j.colcom.2023.100719
• Publication Date: 2023-05-20T10:23:21Z
• Authors: Peng Wang; Jiwen Geng; Chunyu Tan; Qiang Wei
• Keywords: HDAC3

Adhesive interface stiffness is capable of modulating cellular behavior and gene expression, yet the underlying mechanobiological mechanisms remain unclear. In this study, we investigated effects on expression pathways by hydrogels with divergent stiffness. Our results reveal that adhesive affects cytoplasmic mechanotranduction as well nuclear mechanics, ultimately regulating subcellular localization HDAC3. Further investigation unfolds HDAC3 directly global acetylation levels within nucleus. And HDAC3-induced changes are regulated myosin contraction, thereby portraying downstream expression. Additionally, our study indicates stiffness-mediated regulation nuclear-cytoplasmic redistribution dependent CRM1, inhibition CRM1 impedes export summary, work provides an overview how can be manipulated through cell adhesion stiffness, altering upstream RNA polymerase II activity