Non-invasive in situ visualization of the murine cranial vasculature

• DOI: 10.1016/j.crmeth.2021.100151
• Publication Date: 2022-01-24T15:34:41Z
• Authors: Jared S. Rosenblum; Anthony J. Cappadona; Pashayar P. Lookian; Vikram Chandrashe...; Jean-Paul Bryant; Vibhu Chandrashekhar; David Y. Zhao; Russell H. Knutsen; Danielle R. Donahue; Dorian B. McGavern; Beth A. Kozel; John D. Heiss; Karel Pacak; Zhengping Zhuang
• Keywords: Neurovascular bundle; Ray casting; Feature (linguistics)

Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature neurovascular interfaces. To accomplish this, we developed a non-invasive workflow visualize murine via polymer casting of vessels, iterative sample processing micro-computed tomography, automatic deformable image registration, feature extraction, visualization. This methodology is applicable any tissue allows rapid exploration normal altered states.