SEQUIN is an R/Shiny framework for rapid and reproducible analysis of RNA-seq data

• DOI: 10.1016/j.crmeth.2023.100420
• Publication Date: 2023-03-06T15:33:16Z
• Authors: Claire Weber; Marissa B. Hirst; Ben Ernest; Nicholas J. Schaub; Kelli M. Wilson; Ke Wang; Hannah M. Baskir; Pei-Hsuan Chu; Carlos A. Tristan; Ilyas Singeç
• Keywords: Upload; Benchmarking; RNA-Seq; Data set

SEQUIN is a web-based application (app) that allows fast and intuitive analysis of RNA sequencing data derived for model organisms, tissues, single cells. Integrated app functions enable uploading datasets, quality control, gene set enrichment, visualization, differential expression analysis. We also developed the iPSC Profiler, practical module scoring tool helps measure compare pluripotent differentiated cell types. Benchmarking to other commercial non-commercial products underscored several advantages SEQUIN. Freely available public, empowers scientists using interdisciplinary methods investigate present transcriptome firsthand with state-of-the-art statistical methods. Hence, democratize increase throughput interrogating biological questions next-generation single-cell resolution.