Magnesium ions regulate mesenchymal stem cells population and osteogenic differentiation: A fuzzy agent-based modeling approach

• DOI: 10.1016/j.csbj.2021.07.005
• Publication Date: 2021-07-09T09:03:06Z
• Authors: Jalil Nourisa; Berit Zeller-Plum...; Heike Helmholz; Bérengère Luthrin...; Vladimir Ivannikov; Regine Willumeit-...
• Keywords: Magnesium ions; Agent-based simulation; 0303 health sciences; 03 medical and health sciences; Osteogenic differentiation; Mesenchymal stem cells; Fuzzy logic-based approach; Cell population; TP248.13-248.65; Biotechnology; Research Article

Mesenchymal stem cells (MSCs) are proliferative and multipotent that play a key role in the bone regeneration process. Empirical data have repeatedly shown bioregulatory importance of magnesium (Mg) ions MSC growth osteogenesis. In this study, we propose an agent-based model to predict spatiotemporal dynamics population osteogenic differentiation response Mg2+ ions. A fuzzy-logic controller was designed govern decision-making process by predicting four cellular processes proliferation, differentiation, migration, mortality several important factors such as ions, pH, BMP2, TGF-β1. The calibrated using empirical obtained from three sets cell culture experiments. successfully reproduced observations regarding live count, viability, DNA content, differentiation-related markers alkaline phosphate (ALP) osteocalcin (OC). simulation results, agreement with data, showed within 3–6 mM concentration highest stimulation effect on growth. also correctly stimulatory ALP its inhibitory OC early late markers, respectively. Besides, numerical shed light innate differences cultured different experiments terms capacity well sensitivity proposed can be adopted study osteogenesis around Mg-based implants where released due degradation interact local regulate regeneration.