Structure-function relationship between soluble epoxide hydrolases structure and their tunnel network

• DOI: 10.1016/j.csbj.2021.10.042
• Publication Date: 2021-12-13T17:04:12Z
• Authors: Karolina Mitusińska; Piotr Wojsa; Maria Bzówka; Agata Raczyńska; Weronika Bagrowska; Aleksandra Samol; Patryk Kapica; Artur Góra
• Keywords: Lineage (genetic)

Enzymes with buried active sites maintain their catalytic function via a single tunnel or network. In this study we analyzed the functionality of soluble epoxide hydrolases (sEHs) network, by comparing overall enzyme structure tunnel’s shape and size. sEHs were divided into three groups based on usage. The obtained results compared known substrate preferences studied enzymes, as well reported in our other work evolutionary analyses data. network architecture corresponded lineage source organism large differences between enzymes observed from long fragments insertions. This strategy can be used during protein re-engineering process for changes introduction, whereas modification applied fine-tuning enzyme.