Association of glioma CD44 expression with glial dynamics in the tumour microenvironment and patient prognosis

• DOI: 10.1016/j.csbj.2022.09.003
• Publication Date: 2022-09-09T10:02:31Z
• Authors: Zhanxin Du; Yaqing Wang; Jiaqi Liang; Shaowei Gao; Xiaoying Cai; Yu Yu; Zhihui Qi; Jing Li; Yubin Xie; Zhongxing Wang
• Keywords: Osteopontin

Because of the heterogeneity lower-grade gliomas (LGGs), patients show various survival outcomes that are not reliably predicted by histological classification. The tumour microenvironment (TME) contributes to initiation and progression brain LGGs. Identifying potential prognostic markers based on immune stromal components in TME will provide new insights into dynamic modulation these two We applied ESTIMATE calculate ratio from Cancer Genome Atlas database. After combined differential gene expression analysis, protein–protein interaction network construction CD44 was screened as an independent factor subsequently validated utilizing data Chinese Glioma To decipher association glioma cell with cells progression, RT–qPCR, viability wound healing assays were employed determine whether astrocytes enhance migration upregulating expression. Surprisingly, M1 macrophages identified positively correlated CIBERSORT analysis. CD44+ further suggested interact microglia-derived (M1 phenotype) via osteopontin signalling basis single-cell sequencing data. Overall, we found could elevate level cells, enhancing recruitment may promote stemness osteopontin-CD44 signalling. Thus, might coordinate glial activities serve a therapeutic target marker for