MicroRNAs in exhaled breath condensate: A pilot study of biomarker detection for lung cancer

• DOI: 10.1016/j.ctarc.2023.100689
• Publication Date: 2023-02-10T04:40:00Z
• Authors: Divyanjali Rai; Bijay Pattnaik; Sunil Bangaru; Naveen K. Bhatraju; Jaya Tak; Seetu Kashyap; Umashankar Verma; Rohit Vadala; Geetika Yadav; R S Dhaliwal; Anurag Agrawal; Randeep Guleria; Anant Mohan
• Keywords: Exhaled breath condensate; Diagnostic biomarker; Breath gas analysis

Quantitation of microRNAs secreted by lung cells can provide valuable information regarding health. Exhaled breath condensate (EBC) offers a non-invasive way to sample the microRNAs, and could be used as diagnostic tools for cancer. EBC samples from twenty treatment-naïve patients with pathologically confirmed cancer healthy subjects were profiled miRNAs expression. Selected further validated, using quantitative-PCR, in an independent set 10 both groups. A total 78 found significantly upregulated compared control group. Six these shortlisted validation. Of these, miR-31–3p, let7i, miR-449c upregulated, exhibited good discriminatory power. Differential expression quantitated samples, potential tool early diagnosis