Combined single cell and spatial transcriptome analysis reveals hedgehog pathway-related genes as potential therapeutic targets for cervical cancer

• DOI: 10.1016/j.ctarc.2024.100841
• Publication Date: 2024-08-28T17:01:44Z
• Authors: Jing Zheng; Miaomiao Dou; Zhenzhen WU; Chunjie Zhang; Bo Yang; Zhijie Liu; Min Zhang; Fang Wang
• Keywords: Myofibroblast

Cervical cancer (CC) remains one of the most common and deadly malignancies among women worldwide, with exceptionally high morbidity mortality rates. The aberrant activation hedgehog pathway is intimately associated tumor development progression. Nevertheless, potential therapeutic targets within in CC have yet to be clearly identified. In this study, we conducted an in-depth investigation pathway-related genes CC, integrating single-cell sequencing data spatial transcriptomics. Utilizing a comprehensive scoring algorithm, identified that myofibroblasts tissue exhibit highly enriched pathway. Our analysis myofibroblast process revealed MYH9 plays crucial role. Further exploration using transcriptome allowed us delve into role myofibroblasts. We discovered MYH9-negative MYH9-positive display distinct profiles. Validation extensive demonstrated infiltration risk factor for patients, significantly impacting prognosis immunotherapeutic efficacy. study provides unique insights relationship between pathway, offering new directions treatment strategies.