Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss

• DOI: 10.1016/j.cub.2014.09.054
• Publication Date: 2014-11-16T20:11:58Z
• Authors: Tom A. Bennett; Maureen M. Liu; Tsuyoshi Aoyama; Nicole M. Bierfreund; Marion Braun; Yoan Coudert; Ross J. Dennis; Devin O’Connor; Xiao Y. Wang; Chris D. White; Eva L. Decker; Ralf Reski; C. Jill Harrison
• Keywords: Physcomitrella patens; Sporophyte; Bryopsida; Leafy; Polar auxin transport

BackgroundPlant body plans arise by the activity of meristematic growing tips during development and radiated independently in gametophyte (n) sporophyte (2n) stages life cycle evolution. Although auxin its intercellular transport PIN family efflux carriers are primary regulators sporophytic shoot flowering plants, extent conservation function within land plants mechanisms regulating bryophyte gametophytic largely unknown.ResultsWe have found that treating shoots moss Physcomitrella patens with exogenous auxins inhibitors disrupts apical leaf development. Two plasma membrane-targeted proteins expressed leafy shoots, pin mutants resemble treated or inhibitors. PIN-mediated regulates cell function, initiation, shape, tropisms gametophytes. mutant sporophytes sometimes branched, reproducing a phenotype only previously seen fossil record rare natural variants.ConclusionsOur results show is an ancient, conserved regulator