Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development

• DOI: 10.1016/j.cub.2018.11.059
• Publication Date: 2019-01-03T17:00:41Z
• Authors: Emilie W. Olstad; Christa Ringers; Jan N. Hansen; Adinda Wens; Cecilia Brandt; Dagmar Wachten; Emre Yaksi; Nathalie Jurisch-...
• Keywords: Motile cilium; Pulsatile flow; Ventricular system; Lateral ventricles

Motile cilia are miniature, propeller-like extensions, emanating from many cell types across the body. Their coordinated beating generates a directional fluid flow, which is essential for various biological processes, respiration to reproduction. In nervous system, ependymal cells extend their motile into brain ventricles and contribute cerebrospinal (CSF) flow. Although not only contributors CSF functioning crucial, as patients with defects develop clinical features, like hydrocephalus scoliosis. flow was suggested primarily deliver nutrients remove waste, but recent studies emphasized its role in development function. Nevertheless, it remains poorly understood how ciliary organizes fulfill these roles. Here, we study of larval zebrafish. We identified that different populations ciliated spatially organized generate powered by beating. Our investigations revealed confined within individual ventricular cavities, little exchange between ventricles, despite pulsatile displacement caused heartbeat. Interestingly, our results showed boundaries supporting this compartmentalized abolished during bodily movement, highlighting multiple physiological processes regulate hydrodynamics Finally, perturbing reduces hydrodynamic coupling disrupts development. propose motile-cilia-generated crucial regulating distribution ventricles.