Production of interferon gamma and interleukin 17A in chicken T-cell subpopulations hallmarks the stimulation with live, irradiated and killed avian pathogenic Escherichia coli

• DOI: 10.1016/j.dci.2022.104408
• Publication Date: 2022-04-04T05:45:04Z
• Authors: Sina Bagheri; Surya Paudel; Viskam Wijewardana; Richard Thiga Kan...; Giovanni Cattoli; Michael Hess; Dieter Liebhart; Taniya Mitra
• Keywords: Ex vivo

Avian pathogenic Escherichia coli (APEC) causes colibacillosis with different clinical manifestations. The disease is associated compromised animal welfare and results in substantial economic losses poultry production worldwide. So far, immunological mechanisms of protection against are not comprehensively resolved. Therefore, the present study aimed to use an ex vivo model applying chicken mononuclear cells stimulated by live inactivated APEC. For this purpose, 8-color flow cytometry panel was set up target viable immune including CD45+, CD8α+, CD4+, TCR-γδ+, Bu-1+ monocytes/macrophages along cytokines interferon gamma (IFN-γ) or interleukin 17A (IL-17A). applied investigate effect two types APEC (formalin-killed irradiated APEC) on cellular response. that, from spleen, lung blood 10-week-old specific pathogen-free layer birds were isolated live, killed Intracellular cytokine staining RT-qPCR assays for detection IFN-γ IL-17A protein level, as well at mRNA level spleenocytes. Ex stimulation splenocytes, peripheral (PBMCs) chickens showed increasing number producing level. Phenotyping organs revealed that mainly produced TCR-γδ+ T CD4+ following Expression very similar but lower when applied. Consequently, study, using response suggest relevance infection which needs be further investigated primed birds.