The Spike protein of SARS-CoV-2 signals via Tlr2 in zebrafish

• DOI: 10.1016/j.dci.2022.104626
• Publication Date: 2022-12-30T07:14:29Z
• Authors: Sylwia D. Tyrkalska; Alicia Martínez-L...; Annamaria Pedoto; Sergio Candel; María L. Cayuela; Victoriano Mulero
• Keywords: TLR3; TLR7; TLR9; Viral protein

One of the most studied defense mechanisms against invading pathogens, including viruses, are Toll-like receptors (TLRs). Among them, TLR3, TLR7, TLR8 and TLR9 detect different forms viral nucleic acids in endosomal compartments, whereas TLR2 TLR4 recognize structural nonstructural proteins outside cell. Although many TLRs have been shown to be involved SARS-CoV-2 infection detection proteins, studies performed vitro results obtained rather contradictory. In this study, we report using unique advantages zebrafish model for vivo imaging gene editing that S1 domain Spike protein from Wuhan strain (S1WT) induced hyperinflammation larvae via a Tlr2/Myd88 signaling pathway independently interleukin-1β production. addition, S1WT also triggered emergency myelopoiesis, but case through Tlr2/Myd88-independent pathway. These shed light on fish host responses proteins.