A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish

• DOI: 10.1016/j.devcel.2015.01.032
• Publication Date: 2015-03-05T16:46:00Z
• Authors: Julien Ablain; Ellen M. Durand; Song Yang; Yi Zhou; Leonard I. Zon
• Keywords: 0303 health sciences; Erythrocytes; Genetic Vectors; Zebrafish Proteins; Disease Models, Animal; Gene Knockout Techniques; Luminescent Proteins; 03 medical and health sciences; Animals; Clustered Regularly Interspaced Short Palindromic Repeats; Tumor Suppressor Protein p53; Genetic Engineering; Promoter Regions, Genetic; Zebrafish; Developmental Biology; Anemia, Diamond-Blackfan; Red Fluorescent Protein

CRISPR/Cas9 technology of genome editing has greatly facilitated the targeted inactivation of genes in vitro and in vivo in a wide range of organisms. In zebrafish, it allows the rapid generation of knockout lines by simply injecting a guide RNA (gRNA) and Cas9 mRNA into one-cell stage embryos. Here, we report a simple and scalable CRISPR-based vector system for tissue-specific gene inactivation in zebrafish. As proof of principle, we used our vector with the gata1 promoter driving Cas9 expression to silence the urod gene, implicated in heme biosynthesis, specifically in the erythrocytic lineage. Urod targeting yielded red fluorescent erythrocytes in zebrafish embryos, recapitulating the phenotype observed in the yquem mutant. While F0 embryos displayed mosaic gene disruption, the phenotype appeared very penetrant in stable F1 fish. This vector system constitutes a unique tool to spatially control gene knockout and greatly broadens the scope of loss-of-function studies in zebrafish.