RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6

• DOI: 10.1016/j.devcel.2017.04.010
• Publication Date: 2017-05-08T16:32:35Z
• Authors: Charlotte H. Durkin; Flavia Leite; João V. Cordeiro; Yutaka Handa; Yoshiki Arakawa; Ferran Valderrama; Michael Way
• Keywords: RhoC; Cell membrane

RhoA-mediated regulation of myosin-II activity in the actin cortex controls ability cells to contract and bleb during a variety cellular processes, including cell migration division. Cell contraction blebbing also frequently occur as part cytopathic effect seen many different viral infections. We now demonstrate that vaccinia virus protein F11, which localizes plasma membrane, is required for ROCK-mediated from 2 hr post infection. Curiously, F11-induced dependent on RhoC not RhoA signaling ROCK. Moreover, RhoC-driven depends upstream inhibition RhoD by F11. This prevents regulating its downstream effector Pak6, alleviating suppression kinase. Our observations with have demonstrated recruits Pak6 membrane antagonize blebbing.