RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
rho GTP-Binding Proteins
0301 basic medicine
blebbing
610
TYROSINE KINASE
Vaccinia virus
cell contraction
Article
Cell Line
03 medical and health sciences
Cell Movement
Cell Line, Tumor
RhoGTPase crosstalk
Humans
CORTICAL ACTIN
IN-VIVO
11 Medical and Health Sciences
TAIL FORMATION
0303 health sciences
Tumor
Science & Technology
VACCINIA VIRUS-INFECTION
F11L-MEDIATED INHIBITION
ACTIN-BASED MOTILITY
Cell Biology
STRESS FIBERS
RhoC
06 Biological Sciences
RhoD
NUCLEOTIDE EXCHANGE FACTOR
Actins
3. Good health
Pak6
p21-Activated Kinases
rhoC GTP-Binding Protein
PDZ-RHOGEF
rhoA GTP-Binding Protein
Life Sciences & Biomedicine
Developmental Biology
Signal Transduction
DOI:
10.1016/j.devcel.2017.04.010
Publication Date:
2017-05-08T16:32:35Z
AUTHORS (7)
ABSTRACT
RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing.
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CITATIONS (28)
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