Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes

• DOI: 10.1016/j.devcel.2017.09.010
• Publication Date: 2017-10-12T19:44:45Z
• Authors: Kruno Vukušić; Renata Buđa; Agneza Bosilj; Ana Milas; Nenad Pavin; Iva M. Tolić
• Keywords: Astral microtubules

During cell division, mitotic spindle microtubules segregate chromosomes by exerting forces on kinetochores. What drive chromosome segregation in anaphase remains a central question. The current model for human cells includes shortening of kinetochore fibers and separation poles. Both processes require kinetochores to be linked with the Here we show, combining laser ablation, photoactivation, theoretical modeling, that can separate without any attachment one pole. This requires bridging fiber, microtubule bundle connects sister fibers. Bridging fiber intact spindles slide apart fibers, indicating strong crosslinks between them. We conclude sliding within drives pole pushes poleward friction passive these Thus, works together chromosomes.