The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A

• DOI: 10.1016/j.devcel.2018.03.008
• Publication Date: 2018-04-09T11:41:35Z
• Authors: Claudia Puri; Mariella Vicinanza; Avraham Ashkenazi; Matthew J. Gratian; Qifeng Zhang; Carla F. Bento; Maurizio Renna; Fiona M. Menzies; David C. Rubinsztein
• Keywords: Autophagosome; ATG16L1; Compartment (ship)

Autophagy is a critical pathway that degrades intracytoplasmic contents by engulfing them in double-membraned autophagosomes are conjugated with LC3 family members. These membranes specified phosphatidylinositol 3-phosphate (PI3P), which recruits WIPI2, which, turn, ATG16L1 to specify the sites of LC3-conjugation. Conventionally, phosphatidylinositides act concert other proteins targeting effectors specific membranes. Here we describe WIPI2 localizes autophagic precursor binding RAB11A, protein specifies recycling endosomes, and PI3P formed on RAB11A-positive upon starvation. Loss RAB11A impairs recruitment assembly machinery. primary direct platform for canonical autophagosome formation enables autophagy transferrin receptor damaged mitochondria. While this compartment may receive membrane inputs from sources enable biogenesis, appear be evolve.