Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns
Male
0301 basic medicine
CEL-seq
Disorders of Sex Development
mRNA sequencing
germline
sperm
male fertility
VESICLES
03 medical and health sciences
Spatio-Temporal Analysis
NEUROPEPTIDE GENE
5. Gender equality
Journal Article
Animals
Hermaphroditic Organisms
RNA, Messenger
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Gonads
NEURONS
GERM-LINE
Sex Characteristics
0303 health sciences
spatial transcriptomics
Gene Expression Profiling
Ovary
Gene Expression Regulation, Developmental
Nuclear Proteins
Cell Differentiation
NEMATODE
Sex Determination Processes
16. Peace & justice
MALE MATING-BEHAVIOR
Spermatozoa
Sperm
READ ALIGNMENT
FAMILY
Meiosis
Germ Cells
C. elegans
Female
SPERM
DOI:
10.1016/j.devcel.2018.10.016
Publication Date:
2018-11-08T15:40:51Z
AUTHORS (8)
ABSTRACT
To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.
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CITATIONS (64)
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