NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system

• DOI: 10.1016/j.devcel.2022.04.008
• Publication Date: 2022-04-27T17:24:38Z
• Authors: Jocelyn L.Y. Tang; Anna E. Hakes; Robert Krautz; Takumi Suzuki; Esteban G. Contreras; Paul M. Fox; Andrea H. Brand
• Keywords: Technology; NEURAL STEM-CELLS; NanoDam; neural development; 03 medical and health sciences; Neural Stem Cells; Animals; Drosophila Proteins; NKX2.1; Cell Lineage; SPECIFICATION; OUTER SUBVENTRICULAR ZONE; neural stem cells; 0303 health sciences; PROGENITORS; NEUROBLAST COMPETENCE; Brain; Gene Expression Regulation, Developmental; LINEAGE; Drosophila melanogaster; TARGETED GENE-EXPRESSION; CENTRAL COMPLEX; Drosophila; ARX; temporal patterning; RADIAL GLIA; GENERATION

Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II stem cells in the Drosophila central brain produce transit-amplifying intermediate (INPs) that exhibit temporal patterning. However, known factors cannot account diversity adult brain. To search missing factors, we developed NanoDam, which enables rapid genome-wide profiling endogenously tagged proteins vivo with a single genetic cross. Mapping targets transcription NanoDam revealed Homeobrain and Scarecrow (ARX NKX2.1 orthologs) are also factors. We show define middle-aged late INP windows play role cellular longevity. Strikingly, have functions as developing visual system. cell-type-specific resolution easily adapted use higher organisms.