Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport

• DOI: 10.1016/j.devcel.2023.08.030
• Publication Date: 2023-10-09T14:30:59Z
• Authors: Nari Kim; Tae-Hyeon Kim; Chaelim Kim; Jee-Eun Lee; Myeong-Gyun Kang; Sanghee Shin; Minkyo Jung; Jong-Seo Kim; Ji Young Mun; Hyun-Woo Rhee; Seung-Yeol Park; Yongdae Shin; Joo-Yeon Yoo
• Keywords: COPII; COPI; Transport protein; Vesicular Transport Proteins

Newly synthesized proteins in the endoplasmic reticulum (ER) are sorted by coat protein complex II (COPII) at ER exit site en route to Golgi. Under cellular stresses, COPII become targets of regulation control transport. Here, we show that outer Sec31 and Sec13 selectively sequestered into biomolecular condensate SCOTIN/SHISA-5, which interferes with vesicle formation inhibits ER-to-Golgi SCOTIN is an transmembrane a cytosolic intrinsically disordered region (IDR), required essential for condensates. Upon IFN-γ stimulation, condition induces expression condensation, transport was inhibited SCOTIN-dependent manner. Furthermore, cancer-associated mutations perturb its ability form condensates Together, propose impedes through membrane.