Stem cell migration drives lung repair in living mice

• DOI: 10.1016/j.devcel.2024.02.003
• Publication Date: 2024-02-19T15:31:49Z
• Authors: Maurizio Chioccioli; Shuyu Liu; Sumner Magruder; Aleksandra Tata; Lucia Borriello; John E. McDonough; Arvind Konkimalla; Sang-Hun Kim; Jessica Nouws; David G. Gonzalez; Brian Traub; Xianjun Ye; Tao Yang; David R. Entenberg; Smita Krishnaswamy; Caroline E. Hendry; Naftali Kaminski; Purushothama Rao ...; Maor Sauler
• Keywords: Cell type; Ex vivo

Tissue repair requires a highly coordinated cellular response to injury. In the lung, alveolar type 2 cells (AT2s) act as stem replenish both themselves and 1 (AT1s); however, complex orchestration of cell activity after injury is poorly understood. Here, we establish longitudinal imaging AT2s in murine intact tissues ex vivo order track their dynamic behavior over time. We discover that large fraction become motile following provide direct evidence for migration between units. High-resolution morphokinetic mapping further uncovers emergence distinct phenotypes. Inhibition AT2 via genetic depletion ArpC3 leads impaired regeneration AT1s vivo. Together, our results requirement units identify properties motility at high resolution.