Tight junctions control lumen morphology via hydrostatic pressure and junctional tension

• DOI: 10.1016/j.devcel.2024.07.016
• Publication Date: 2024-08-12T14:34:34Z
• Authors: Markus Mukenhirn; Chen-Ho Wang; Tristan Guyomar; Matthew J. Bovyn; Michael F. Staddon; Rozemarijn E. van...; Riccardo Maraspini; Linjie Lu; Cecilie Martin-Le...; Masaki Sano; Martin Lehmann; Tetsuya Hiraiwa; Daniel Riveline; Alf Honigmann
• Keywords: Lumen (anatomy); Paracellular transport; Hydrostatic pressure

Formation of fluid-filled lumina by epithelial tissues is essential for organ development. How cells control the hydraulic and cortical forces to lumen morphology not well understood. Here, we quantified mechanical role tight junctions in formation using MDCK-II cysts. We found that paracellular ion barrier formed claudin receptors required inflation a lumen. However, depletion zonula occludens scaffold resulted collapse folding apical membranes. Combining quantitative measurements hydrostatic pressure junctional tension with modeling enabled us explain morphologies from pressure-tension force balance. Tight promote decreasing via inhibition myosin. In addition, our results suggest excess area contributes opening. Overall, provide understanding how use modulate tissue shape.