Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting
Microbiological Techniques
0301 basic medicine
0303 health sciences
Sputum
Microbial Sensitivity Tests
Mycobacterium tuberculosis
DNA Fingerprinting
Specimen Handling
3. Good health
03 medical and health sciences
Cost of Illness
Tuberculosis, Multidrug-Resistant
Equipment Contamination
Health Resources
Humans
Tuberculosis
DOI:
10.1016/j.diagmicrobio.2006.03.009
Publication Date:
2006-05-07T11:12:53Z
AUTHORS (13)
ABSTRACT
One obstacle to wider use of rapid liquid culture-based tuberculosis diagnostics such as the microscopic observation drug susceptibility (MODS) assay is concern about cross-contamination. We investigated the rate of laboratory cross-contamination in MODS, automated MBBacT, and Lowenstein-Jensen (LJ) cultures performed in parallel, through triangulation of microbiologic (reculturing stored samples), molecular (spoligotype/RFLP), and clinical epidemiologic data. At least 1 culture was positive for Mycobacterium tuberculosis for 362 (11%) of 3416 samples; 53 were regarded as potential cross-contamination suspects. Cross-contamination accounted for 17 false-positive cultures from 14 samples representing 0.41% (14/3416) and 0.17% (17/10248) of samples and cultures, respectively. Positive predictive values for MODS, MBBacT (bioMérieux, Durham, NC), and LJ were 99.1%, 98.7%, and 99.7%, and specificity was 99.9% for all 3. Low rates of cross-contamination are achievable in mycobacterial laboratories in resource-poor settings even when a large proportion of samples are infectious and highly sensitive liquid culture-based diagnostics such as MODS are used.
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