Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

• DOI: 10.1016/j.diagmicrobio.2018.03.004
• Publication Date: 2018-03-12T13:31:34Z
• Authors: Caroline R. Espada; Paola A. Ortiz; Jeffrey J. Shaw; Aldina M.P. Barral; Jackson M.L. Costa; Silvia R.B. Uliana; Adriano C. Coelho
• Keywords: Leishmania; Protozoan Proteins; Leishmaniasis, Cutaneous; Sequence Analysis, DNA; DNA, Protozoan; Polymerase Chain Reaction; Leishmania braziliensis; 03 medical and health sciences; 0302 clinical medicine; Animals; Humans; HSP70 Heat-Shock Proteins; Genome, Protozoan; Alleles; Brazil; Phylogeny; Polymorphism, Restriction Fragment Length

Hsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leishmania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L. (V.) lindenbergi were very similar to those of other L. (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil.