The effects of TPT and dietary quercetin on growth, hepatic oxidative damage and apoptosis in zebrafish

• DOI: 10.1016/j.ecoenv.2021.112697
• Publication Date: 2021-08-25T00:36:00Z
• Authors: Chunnuan Zhang; Dongxue Jiang; Junhui Wang; Qian Qi
• Keywords: Inflammation; Environmental sciences; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; TD172-193.5; Oxidative stress; Triphenyltin; Quercetin; Apoptosis; GE1-350; Environmental pollution

The objective of this study was to determine the effects of triphenyltin (TPT) and dietary quercetin on the growth, oxidative stress and apoptosis in zebrafish. A total of 240 fish were divided into 4 groups with three replicates as follows: fish were fed with the basal diet as the control group (D1), only 10 ng/L TPT (D2), 10 ng/L TPT + 100 mg/kg quercetin (D3), and only 100 mg/Kg quercetin as the D4 group. At the end of the study period (56 d), the results showed that the growth performance of the fish that were fed 100 mg/kg quercetin was significantly higher than that of fish that were exposed to 10 ng/L TPT. Quercetin ameliorated oxidative stress, which decreased malondialdehyde (MDA) and nitric oxide (NO) levels and improved antioxidant enzyme activities. The mRNA expressions of the key apoptotic gene and pro-inflammatory cytokines were significantly induced by TPT exposure. However, dietary quercetin prevented a marked increase in the Bax, caspase3 and caspase9 transcript abundances that were induced by TPT. In addition, the quercetin treatments decreased inflammation by regulating the NF-kB signalling pathway. In conclusion, our findings suggested that TPT induced oxidative stress and apoptosis in zebrafish and that the pretreatment with quercetin showed an ameliorative role. Dietary 100 mg/ kg quercetin helps to prevent oxidative damage, apoptosis and inflammation in TPT treated zebrafish.