Synthesis, cellular uptake, apopotosis, cytotoxicity, cell cycle arrest, interaction with DNA and antioxidant activity of ruthenium(II) complexes

• DOI: 10.1016/j.ejmech.2011.04.049
• Publication Date: 2011-04-30T21:50:53Z
• Authors: Hong-Liang Huang; Zheng-Zheng Li; Zhen-Hua Liang; Jun-Hua Yao; Yun-Jun Liu
• Keywords: Differential Thermal Analysis; Cell Cycle; Liver Neoplasms; Antineoplastic Agents; Apoptosis; Electrophoretic Mobility Shift Assay; DNA; Flow Cytometry; Ligands; 01 natural sciences; Antioxidants; Intercalating Agents; Ruthenium; 0104 chemical sciences; 2,2'-Dipyridyl; Coordination Complexes; Cell Line, Tumor; Humans; DNA Cleavage; Phenanthrolines; Plasmids

A new ligand and two ruthenium(II) complexes [Ru(bpy)(2)(DNPIP)](ClO(4))(2)1 and [Ru(bpy)(2)(DAPIP)](ClO(4))(2)2 were synthesized and characterized. The DNA-binding constants for complexes 1 and 2 were determined to be 2.24 (±0.30) × 10(5) M(-1) (s = 1.29) and 6.34 (±0.32) × 10(4) M(-1) (s = 2.84). The photocleavage of pBR322 DNA by Ru(II) complexes was investigated. The cytotoxicity of complexes 1 and 2 were assessed against three tumor cell lines. The apoptosis and cellular uptake were studied. The retardation assay of pGL 3 plasmid DNA was explored. The cell cycle arrest was analysized by flow cytometry. The antioxidant activities of the ligand and complexes were also investigated.