Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarine-derived ligand

• DOI: 10.1016/j.ejmech.2013.12.051
• Publication Date: 2014-01-21T01:16:04Z
• Authors: Dragoslav R. Ilić; Verica V. Jevtić; Gordana P. Radić; Katarina Arsikin; Biljana Ristić; Ljubica Harhaji-T...; Nenad Vuković; Slobodan Sukdolak; Olivera Klisurić; Vladimir Trajković; Srećko R. Trifunović
• Keywords: Membrane Potential, Mitochondrial; Magnetic Resonance Spectroscopy; Crystallography, X-Ray; Ligands; 01 natural sciences; 3. Good health; 0104 chemical sciences; Enzyme Activation; Mice; Coumarins; Caspases; Cell Line, Tumor; Animals; Humans; Drug Screening Assays, Antitumor; Reactive Oxygen Species; Palladium

The new coumarine derivative, 3-(1-(2-hydroxyethylamino)ethylidene)chroman-2,4--dione, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, (1)H and (13)C NMR spectroscopy. The proposed structure of the complex was confirmed on the basis of the X-ray structural study. The palladium(II) complex decreased viability of L929 mouse fibrosarcoma, U251 human glioma and B16 mouse melanoma cell lines in a dose dependent manner, while its ligand exhibited no significant cytotoxicity. The cytotoxic effect of the complex was comparable to that of cisplatin, and mediated by apoptosis associated with oxidative stress, mitochondrial depolarization and caspase activation. Therefore, our results indicate that newly synthesized palladium(II) complex might be a potential candidate for anticancer therapy.