Synthesis, crystal structure and antitumor effect of a novel copper(II) complex bearing zoledronic acid derivative

• DOI: 10.1016/j.ejmech.2014.10.028
• Publication Date: 2014-10-13T13:12:10Z
• Authors: Ling Qiu; Gaochao Lv; Liubin Guo; Liping Chen; Shineng Luo; Meifen Zou; Jianguo Lin
• Keywords: Models, Molecular; Diphosphonates; Dose-Response Relationship, Drug; Molecular Structure; Cell Survival; Cell Cycle; Imidazoles; Antineoplastic Agents; Hep G2 Cells; Crystallography, X-Ray; HCT116 Cells; Zoledronic Acid; 01 natural sciences; 3. Good health; 0104 chemical sciences; Structure-Activity Relationship; Cell Line, Tumor; Organometallic Compounds; Humans; Drug Screening Assays, Antitumor; Copper; Cell Proliferation

A great majority of Cu(II) complexes currently studied in the anticancer research field exert their antiproliferative activities through ligand exchange. In this work, we present the synthesis and structural characterization of two novel Cu(II) complexes, {[Cu3(ZL)2(H2O)6]·6H2O}n (1) (ZL = 1-hydroxy-2-(1H-imidazol-1-yl)ethane-1,1-diyldiphosphonic acid) and [Cu(IPrDP)2]·3H2O (2) (IPrDP = 1-hydroxy-3-(1H-imidazol-1-yl)propane-1,1-diyldiphosphonic acid). Due to the insolubility of polymer 1 in common solvents, only the biological activities of complex 2 were investigated. The antitumor activity of complex 2 was evaluated against a panel of human cancer cell lines, including U2OS, A549, HCT116, MDA-MB-231 and HepG2. Complex 2 exhibited comparable cytotoxic effect to cisplatin (CDDP) against the human colon carcinoma cells HCT116, and superior selectivity for inhibiting human hepatocarcinoma cells rather than normal liver cells. The cell cycle distribution analysis indicates that complex 2 inhibits human carcinoma cells by inducing the cell cycle arrest at the G2/M phase, showing a similar mechanism of action to that of CDDP. The binding interaction of complex 2 with calf thymus DNA (CT-DNA) has been explored by UV-vis absorption and circular dichroism (CD), demonstrating complex 2 has a moderate binding affinity for DNA through intercalation.